Hamamatsu’s ImagEM cameras (C9100-13 and C9100-14) offer high-performance imaging for virtually any application in optical microscopy. Whether you’re capturing ultra-low-light images that require long integration times or real-time, high frame rate imaging of low-light fluorescence, you can rely on the ImagEM to provide high sensitivity, exceptional stability, and extremely low noise.
This digital video presents a cameleon biosensor composed of cyan and yellow fluorescent proteins sandwiching a calmodulin domain and the m13 domain is being expressed transiently in human cervical carcinoma epithelial cells (HeLa line). In the presence of increasing levels of intracellular calcium, the M13 domain binds the calmodulin peptide to produce an increase in FRET between the fluorescent proteins, visualized in the videos as a change in color from cyan to red. Video 1 - Run Time: 6 Seconds.
The digital video illustrates the interplay between mitochondria (labeled with DsRed fluorescent protein) and the microtubules by examining the tracking of microtubule +TIPs (plus end tracking proteins) labeled with EB3 fused to the popular tracking probe, mEGFP. The length of microtubules in the cell varies between 200 nanometers and 25 micrometers, depending upon the task of a particular microtubule and the state of the cell's life cycle. Microtubules are believed to act as a transport mechanism for mitochondria. EB3 is a protein that binds to the plus-ends of microtubules and can be visualized tracking through the cytoplasm.
The fluorescent protein known as mEmerald is a high-performance version of EGFP that is significantly brighter and also matures faster. When coupled to the microtubule end-binding protein EB3, traces of the fluorescently labeled positive microtubule ends can be observed traversing the cytoplasm and forming the spindle during mitosis. Compare these digital videos with those of EGFP-tubulin presented in the section above.
Hamamatsu Photonics K.K. (Japan) is a leading manufacturer of devices for the generation and measurement of UV, visible, and infrared light. These devices include photomultiplier tubes, photodiodes, image sensors, scientific CCD cameras, and light sources. Hamamatsu also manufactures a variety of X-ray solutions as well as specialized photonic systems. We are committed to the advancement of photonics through extensive research.
